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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha : |
02/02/2022 |
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Actualizado : |
02/02/2022 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
TORRES, D.; DELGADO-CERRONE, L.; LUNA, L.; RESQUÍN, F.; AGUILAR, A.V.; SEBENN, A.M. |
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Afiliación : |
DIEGO GABRIEL TORRES DINI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; LEONARDO DELGADO-CERRONE, Instituto de Investigaciones Biológicas Clemente Estable (IIBCE), Av. Italia 3318, 11600 Montevideo, Uruguay.; LORENA LUNA, Centro Universitario de Tacuarembó (CUT), Ruta 5 Km 386, CEP 45000, Tacuarembó, TB, Uruguay; JOSE FERNANDO RESQUIN PEREZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ANANDA VIRGINIA AGUIAR, Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA), Centro Nacional de Pesquisa de Florestas, Estrada da Ribeira, Km 111 - Bairro Guaraituba, 83411-000, Colombo, PR, Brazil.; ALEXANDRE MAGNO SEBBENN, Instituto Florestal de São Paulo, CP 1322, São Paulo, SP, 01059-970, Brazil. |
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Título : |
The traceability of Eucalyptus clones using molecular markers. |
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Fecha de publicación : |
2021 |
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Fuente / Imprenta : |
Silvae Genetica, 2021, 70(1) 217-225. OPEN ACCESS. https://doi.org/10.2478/sg-2021-0019 |
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DOI : |
10.2478/sg-2021-0019 |
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Idioma : |
Inglés |
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Notas : |
Article history: Published Online 25 Dec 2021.
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.
Corresponding author: Diego Torres-Dini, E-mail: diego.torres.dini@gmail.com |
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Contenido : |
ABSTRACT.- The improvement of Eucalyptus clones plays a crucial role in modern silviculture. This study used a set of 17 microsatellite loci to analyze the genetic diversity and structure of 107 elite clones (80 E. grandis and 27 E. globulus). All clones were cultivated in Uruguay and were sourced from three different providers. Using the fingerprinting technique, an exclusive molecular profile was assigned for each clone, and the genotyping reaction showed differences between the two species. The cumulative probability of identifying two random individuals that share the same genotype (???? ) with all 17 loci, was estimated as low for E. grandis (1.18×10-15) and E. globulus (4.03×10-14). The combined PIsibs was (1.05×10-5) and (2.17×10-5) for E. grandis and E. globulus, respectively. A total of 180 alleles were detected for E. grandis and 100 for E. globulus. We found a high mean number of alleles per locus (10 for E. grandis and 6 for E. globulus), and the results for mean polymorphic information content ( PIC ) were (0.648) and (0.548), respectively. The observed heterozygosity ( Ho ) ranged from 0.216 to 0.838 (mean = 0.509) for E. grandis and 0 to 1 (mean = 0.566) for E. globulus. Two core sets of seven EST-SSR loci were identified for each species. These markers revealed unambiguous fragment amplification, providing a minimum number of SSRs for effective clonal identification. The genetic structure analysis suggests that the germplasm of the E. grandis population is structured in four clusters, while the E. globulus population consists of two clusters.
© 2020 Diego Torres-Dini et al., published by Sciendo MenosABSTRACT.- The improvement of Eucalyptus clones plays a crucial role in modern silviculture. This study used a set of 17 microsatellite loci to analyze the genetic diversity and structure of 107 elite clones (80 E. grandis and 27 E. globulus). All clones were cultivated in Uruguay and were sourced from three different providers. Using the fingerprinting technique, an exclusive molecular profile was assigned for each clone, and the genotyping reaction showed differences between the two species. The cumulative probability of identifying two random individuals that share the same genotype (???? ) with all 17 loci, was estimated as low for E. grandis (1.18×10-15) and E. globulus (4.03×10-14). The combined PIsibs was (1.05×10-5) and (2.17×10-5) for E. grandis and E. globulus, respectively. A total of 180 alleles were detected for E. grandis and 100 for E. globulus. We found a high mean number of alleles per locus (10 for E. grandis and 6 for E. globulus), and the results for mean polymorphic information content ( PIC ) were (0.648) and (0.548), respectively. The observed heterozygosity ( Ho ) ranged from 0.216 to 0.838 (mean = 0.509) for E. grandis and 0 to 1 (mean = 0.566) for E. globulus. Two core sets of seven EST-SSR loci were identified for each species. These markers revealed unambiguous fragment amplification, providing a minimum number of SSRs for effective clonal identification. The genetic structure analysis suggests that the germplasm of the E. grandis population is st... Presentar Todo |
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Palabras claves : |
Clone; Clone certification; Genotyping; Identity; Multiplex; Nurseries; Traceability. |
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Asunto categoría : |
K01 Ciencias forestales - Aspectos generales |
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URL : |
https://sciendo.com/pdf/10.2478/sg-2021-0019
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Marc : |
LEADER 02660naa a2200289 a 4500
001 1062729
005 2022-02-02
008 2021 bl uuuu u00u1 u #d
024 7 $a10.2478/sg-2021-0019$2DOI
100 1 $aTORRES, D.
245 $aThe traceability of Eucalyptus clones using molecular markers.$h[electronic resource]
260 $c2021
500 $aArticle history: Published Online 25 Dec 2021. This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License. Corresponding author: Diego Torres-Dini, E-mail: diego.torres.dini@gmail.com
520 $aABSTRACT.- The improvement of Eucalyptus clones plays a crucial role in modern silviculture. This study used a set of 17 microsatellite loci to analyze the genetic diversity and structure of 107 elite clones (80 E. grandis and 27 E. globulus). All clones were cultivated in Uruguay and were sourced from three different providers. Using the fingerprinting technique, an exclusive molecular profile was assigned for each clone, and the genotyping reaction showed differences between the two species. The cumulative probability of identifying two random individuals that share the same genotype (???? ) with all 17 loci, was estimated as low for E. grandis (1.18×10-15) and E. globulus (4.03×10-14). The combined PIsibs was (1.05×10-5) and (2.17×10-5) for E. grandis and E. globulus, respectively. A total of 180 alleles were detected for E. grandis and 100 for E. globulus. We found a high mean number of alleles per locus (10 for E. grandis and 6 for E. globulus), and the results for mean polymorphic information content ( PIC ) were (0.648) and (0.548), respectively. The observed heterozygosity ( Ho ) ranged from 0.216 to 0.838 (mean = 0.509) for E. grandis and 0 to 1 (mean = 0.566) for E. globulus. Two core sets of seven EST-SSR loci were identified for each species. These markers revealed unambiguous fragment amplification, providing a minimum number of SSRs for effective clonal identification. The genetic structure analysis suggests that the germplasm of the E. grandis population is structured in four clusters, while the E. globulus population consists of two clusters. © 2020 Diego Torres-Dini et al., published by Sciendo
653 $aClone
653 $aClone certification
653 $aGenotyping
653 $aIdentity
653 $aMultiplex
653 $aNurseries
653 $aTraceability
700 1 $aDELGADO-CERRONE, L.
700 1 $aLUNA, L.
700 1 $aRESQUÍN, F.
700 1 $aAGUILAR, A.V.
700 1 $aSEBENN, A.M.
773 $tSilvae Genetica, 2021, 70(1) 217-225. OPEN ACCESS. https://doi.org/10.2478/sg-2021-0019
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Registro original : |
INIA Las Brujas (LB) |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas; INIA Tacuarembó. |
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Fecha actual : |
21/02/2014 |
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Actualizado : |
22/02/2014 |
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Autor : |
Díaz, R.Quincke, A.Morón, A.Sawchik, J.Ibáñez, V.Balzarini, M. |
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Título : |
Efecto de la degradación del carbono orgánico del suelo en la productividad potencial de los cultivos |
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Fecha de publicación : |
2009 |
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Fuente / Imprenta : |
ln: Morón, A.; coord. Efectos de la agricultura, la lechería y la ganadería en el recurso natural suelo: impactos y propuestas: resúmenes expandidos. Montevideo (Uruguay): INIA, 2009. |
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Páginas : |
p. 69-75 |
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Serie : |
(INIA Serie Actividades de Difusión ; 587) |
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Idioma : |
Español |
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Notas : |
INIA La Estanzuela |
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Thesagro : |
CARBONO; CULTIVO; SUELO. |
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Asunto categoría : |
-- |
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URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/564/1/112761270809100748.pdf
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Marc : |
LEADER 00740naa a2200181 a 4500
001 1008384
005 2014-02-22
008 2009 bl uuuu u00u1 u #d
100 1 $aDÍAZ, R.QUINCKE, A.MORÓN, A.SAWCHIK, J.IBÁÑEZ, V.BALZARINI, M.
245 $aEfecto de la degradación del carbono orgánico del suelo en la productividad potencial de los cultivos
260 $c2009
300 $ap. 69-75
490 $a(INIA Serie Actividades de Difusión ; 587)
500 $aINIA La Estanzuela
650 $aCARBONO
650 $aCULTIVO
650 $aSUELO
773 $tln: Morón, A.; coord. Efectos de la agricultura, la lechería y la ganadería en el recurso natural suelo: impactos y propuestas: resúmenes expandidos. Montevideo (Uruguay): INIA, 2009.
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